Design and development of synthetic peptide antigens
نویسندگان
چکیده
Using hydrophilicity, surface probability, backbone flexibility and secondary structure parameters, antigenic sites of R-thymosins (TR), ubiquitin and gp41 envelope glycoprotein fragments of HIV1 are determined. Corresponding antigenic sites of TR,, TR,, ubiquitin and gp41 (583-623) are produced either by tryptic cleavage from the natural peptides or by solid phase peptide synthesis (FmocBu' strategy, Ecosyn P batch synthesizer, EppendorfBiotronik, Maintal, Germany). The predicted antigenic fragments allow the generation of specific antibodies for the determination of body fluid or tissue levels and immunostaining experiments, or, in the case of gp41-HIV fragments, the antigens can be used directly for the diagnosis of AIDS patients.
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